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Primers used in RT-PCR for PEDV spike, ORF3, and membrane genes.
| Primer | Nucleotide sequence (5′-3′) | Size of product (bp) |
|---|---|---|
| S-F | TTCTGAGTCACGAACAGCCA | 651 |
| S-R | CATATGCAGCCTGCTCTGAA | |
| ORF3-F | TCCTAGACTTCAACCTTACG | 833 |
| ORF3-R | GGTGACAAGTGAAGCACAGA | |
| M-F | GTCTTACATGCGAATTGACC | 808 |
| M-R | GGCATAGAGAGATAATGGCA |
