Molecular characterization of G-CSF-mobilized CD34+ cells for VEGFR2, VEGF-A and eNOS. (A) PCR amplification products prepared from MNC (G, P) and sorted CD34+ cells (S). G, sample with good mobilized CD34+ cell yield; P, sample with poor mobilized CD34+ cell yield; H, HUVEC cells, positive control. (B) VEGFR2, VEGF-A and eNOS gene expression relative to 18S rRNA by sorted CD34+ cells from six G-CSF-mobilized patients and HUVEC cells as a control. Both figures show that mobilized MNC and sorted CD34+ cells significantly expressed VEGF-A but not VEGFR2 and eNOS.