Fig. 1.

Microscopic observation of C6/36 cells: mock-infected cells (A; 400×), or after infection (day 3) with CTFV strain 153 (B; 400×) or CFAV (C; 200×). (D) Transmission electron micrograph of a thin section of C6/36 cells infected (day 2 post-infection) with CTFV strain 153 (thin arrows) showing multiple round, enveloped viral particles with an electron dense core (thick arrow) accumulated in enlarged cytoplasmic vesicles (scale bar, 200 nm). (E) Kinetics of CTFV₁₅₃ RNA detection in C6/36 infected and mock-infected cells. At different times after infection, total RNA was extracted from the culture supernatant (S) and cell-sediment (C). After reverse-transcription, a virus specific fragment was amplified with primers SeqC and SeqD (supplementary Table 1). The GeneRuler 1 kb Plus DNA ladder (Fermentas, Vilnius, Lithuania) was used as a molecular weight marker.