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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cancer Prev Res (Phila). 2013 Nov 19;7(1):150–160. doi: 10.1158/1940-6207.CAPR-13-0263

Figure 4. ERK1/2 is phosphorylated downstream of Snail and upstream of SPARC.

Figure 4

(A) The cell lines HBEC2, HBEC3, HBEC7, and H3mutP53/KRAS (H3mut) with and without Snail overexpression (-V/-S) were treated with the MEK1/2 phosphorylation inhibitor U0126 (15uM) and evaluated for SPARC protein expression. Membranes were incubated with antibodies against Snail, pERK, total ERK1/2, SPARC, and α-tubulin. Protein levels were normalized to α-tubulin. (B) SPARC mRNA expression was evaluated following U0126 treatment as in (A). mRNA expression was normalized to GUSB. (C) miR-29b miRNA expression was evaluated following U0126 treatment as in (A). miRNA expression was normalized to RNU6b. (** = p < 0.001; *** = p < 0.0001)