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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cancer Prev Res (Phila). 2013 Nov 19;7(1):150–160. doi: 10.1158/1940-6207.CAPR-13-0263

Figure 5. miR-29b is downregulated in NSCLC cell lines overexpressing Snail.

Figure 5

(A) Total RNA was isolated from HBEC3-V/S, HBEC4-V/S, and H3mut-V/S cell lines. Expression levels of miR-29b were evaluated by q-RT-PCR using TaqMan primers. miRNA levels were normalized to RNU6b. (B) Total RNA was isolated from A549-V/S, H1437-V/S, and H292-V/S cell lines. Expression levels of miR-29b were evaluated by q-RT-PCR using TaqMan primers. miRNA levels were normalized to RNU6b. (C) The HBEC lines in (A) were stably transfected with a miR-29b precursor sequence and evaluated for expression of Snail and SPARC. Protein levels were normalized to α-tubulin. (D) We propose a regulatory pathway wherein Snail upregulates TGF-β in an autocrine or paracrine fashion, leading to activation of the MEK/ERK pathway, downregulation of miR-29b, and finally upregulation of SPARC. Snail may suppress miR-29b in both an ERK-dependent or -independent manner. (** = p < 0.001; *** = p < 0.0001)