Skip to main content
. Author manuscript; available in PMC: 2015 Jan 30.
Published in final edited form as: Brain Res. 2013 Dec 16;1545:12–22. doi: 10.1016/j.brainres.2013.12.008

Figure 2.

Figure 2

Immunofluorescent characterization of REEP1 mAb

HEK293A cells were transfected with either Flag-REEP1 or native, untagged-REEP1 cDNAs and analyzed by immunofluorescent wide-field microscopy with anti-Flag mAb (M2) and a new REEP1 mAb respectively. Cells were counterstained with either MitoTracker Red CMXROS or anti-calreticulin antisera, as described in Experimental Procedures. A. Immunofluorescent staining for Flag-REEP1 (M2 mAb) expressed in HEK293A cells revealed a fine reticular network that demonstrated a different localization pattern than mitochondria counterstained with MitoTracker Red CMXROS. B. REEP1 mAb detected a similar reticular staining pattern for untagged-REEP1, as seen above for Flag-REEP1. Mitochondria were counterstained with MitoTracker Red CMXROS. C. The fine reticular immunofluorescent pattern seen with M2 staining of Flag-REEP1 showed a similar localization as the ER marker protein calreticulin. D. REEP1 mAb delineated a similar reticular pattern for untagged-REEP1, when compared with anti-calreticulin staining. Bar = 10 μm. Representative of three transfections.