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. Author manuscript; available in PMC: 2014 Feb 10.
Published in final edited form as: Nat Protoc. 2013 May 16;8(6):1114–1124. doi: 10.1038/nprot.2013.068

Figure 2. Microinjection sites.

Figure 2

The hindbrain (HB) ventricle can be injected directly, or injection into the hindbrain may be performed via penetration through the forebrain (FB). The optimal time for hindbrain and forebrain injection is 30 h.p.f. In contrast, the caudal vein (CV) can be injected between 30 and 48 h.p.f.