Table 1.
Chemical Composition of HDL Subfractions H1 through H5 Obtained From Healthy Normolipidemic Donors (n=6)
| Subfraction | Fraction Distribution (%) | Total Protein (%) | PL (%) | TG (%) | FC (%) | CE (%) | TC (%) |
|---|---|---|---|---|---|---|---|
| H1 | 59.9 ± 2.5 | 54.1 ± 6.9 | 23.5 ± 4.7 | 2.9 ± 2.2 | 3.2 ± 1.5 | 16.3 ± 3.7 | 19.5 ± 4.9 |
| H2 | 13.1 ± 1.4 | 58.5 ± 3.8 | 20.9 ± 2.8 | 1.9 ± 0.6 | 2.3 ± 0.7 | 16.4 ± 2.9 | 18.7 ± 3.6 |
| H3 | 15.5 ± 1.5 | 57.2 ± 2.5 | 18.4 ± 1.4 | 6.4 ± 3.6 | 0.9 ± 0.7a | 17.1 ± 3.1 | 18.0 ± 3.6 |
| H4 | 3.8 ± 0.4 | 56.9 ± 4.5 | 20.5 ± 4.5 | 7.4 ± 4.1b | 1.2 ± 1.3a | 14.1 ± 2.1 | 15.2 ± 3.2 |
| H5 | 7.7 ± 1.0 | 55.8 ± 6.9 | 17.3 ± 6.7 | 8.5 ± 4.6a,b | 2.3 ± 1.8 | 16.1 ± 2.5 | 18.4 ± 4.2 |
Data expressed as mean ± SD. PL, phospholipids; TG, triglycerides; FC, free cholesterol; CE, cholesteryl ester; TC, total cholesterol. The distributions of fraction components were compared by using one-way analysis of variance with the Student-Newman-Keuls post hoc test.
P<0.05 vs H1
P<0.05 vs H2. The percentage of total protein, PL, TG, and TC was determined by dividing each respective amount by the total combined amount in each subfraction. Thus, the sum of the percentages of total protein, PL, TG, and TC add up to 100%. TC (%) is equal to the sum of FC (%) and CE (%). The fraction distribution represents the integrated area of each subfraction on fast protein liquid chromatography divided by that of all fractions.