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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Oct;81(20):6501–6505. doi: 10.1073/pnas.81.20.6501

Detection and immunochemical characterization of a primate type C retrovirus-related p30 protein in normal human placentas.

L B Jerabek, R C Mellors, K B Elkon, J W Mellors
PMCID: PMC391952  PMID: 6093103

Abstract

We find that 12 of 14 specimens of normal human term placentas analyzed by one- or two-dimensional electrophoresis and immunoblotting contain a protein or polypeptide of approximately equal to 30,000 daltons that is antigenically cross-reactive with p30 core protein of the simian sarcoma-associated virus/gibbon ape leukemia virus primate retrovirus group and is physicochemically similar to reference murine and primate type C retrovirus p30s. This finding may lead to an understanding of endogenous type C retrovirus gene expression in humans.

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Selected References

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  1. Barbacid M., Long L. K., Aaronson S. A. Major structural proteins of type B, type C, and type D oncoviruses share interspecies antigenic determinants. Proc Natl Acad Sci U S A. 1980 Jan;77(1):72–76. doi: 10.1073/pnas.77.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benveniste R. E., Callahan R., Sherr C. J., Chapman V., Todaro G. J. Two distinct endogenous type C viruses isolated from the asian rodent Mus cervicolor: conservation of virogene sequences in related rodent species. J Virol. 1977 Mar;21(3):849–862. doi: 10.1128/jvi.21.3.849-862.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benveniste R. E., Lieber M. M., Livingston D. M., Sherr C. J., Todaro G. J., Kalter S. S. Infectious C-type virus isolated from a baboon placenta. Nature. 1974 Mar 1;248(5443):17–20. doi: 10.1038/248017a0. [DOI] [PubMed] [Google Scholar]
  4. Bonner T. I., O'Connell C., Cohen M. Cloned endogenous retroviral sequences from human DNA. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4709–4713. doi: 10.1073/pnas.79.15.4709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bryant M. L., Sherr C. J., Sen A., Todaro G. J. Molecular diversity among five different endogenous primate retroviruses. J Virol. 1978 Oct;28(1):300–313. doi: 10.1128/jvi.28.1.300-313.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  7. Cambiaso C. L., Goffinet A., Vaerman J. P., Heremans J. F. Glutaraldehyde-activated aminohexyl- derivative of Sepharose 4B as a new verstile immunoabsorbent. Immunochemistry. 1975 Apr;12(4):273–278. doi: 10.1016/0019-2791(75)90175-5. [DOI] [PubMed] [Google Scholar]
  8. Dalton A. J., Hellman A., Kalter S. S., Helmke R. J. Ultrastructural comparison of placental virus with several type-c- oncogenic viruses. J Natl Cancer Inst. 1974 Apr;52(4):1379–1381. doi: 10.1093/jnci/52.4.1379. [DOI] [PubMed] [Google Scholar]
  9. Derks J. P., Hofmans L., Bruning H. W., von Rood J. J. Synthesis of a viral protein with molecular weight of 30,000 (p30) by leukemic cells and antibodies cross-reacting with Simian sarcoma virus p30 in serum of a chronic myeloid leukemia patient. Cancer Res. 1982 Feb;42(2):681–686. [PubMed] [Google Scholar]
  10. Elkon K. B. Isoelectric focusing of human IgA and secretory proteins using thin layer agarose gels and nitrocellulose capillary blotting. J Immunol Methods. 1984 Feb 10;66(2):313–321. doi: 10.1016/0022-1759(84)90343-0. [DOI] [PubMed] [Google Scholar]
  11. Geering G., Hardy W. D., Jr, Old L. J., de Harven E., Brodey R. S. Shared group-specific antigen of murine and feline leukemia viruses. Virology. 1968 Dec;36(4):678–680. doi: 10.1016/0042-6822(68)90199-2. [DOI] [PubMed] [Google Scholar]
  12. Geering G., Old L. J., Boyse E. A. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J Exp Med. 1966 Oct 1;124(4):753–772. doi: 10.1084/jem.124.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gilden R. V., Oroszlan S., Huebner R. J. Coexistence of intraspecies and interspecies specific antigenic determinants on the major structural polypeptide of mammalian C-type viruses. Nat New Biol. 1971 May 26;231(21):107–108. doi: 10.1038/newbio231107a0. [DOI] [PubMed] [Google Scholar]
  14. Gilden R. V., Toni R., Hanson M., Bova D., Charman H. P., Oroszlan S. Immunochemical studies of the major internal polypeptide of woolly monkey and gibbon ape type C viruses. J Immunol. 1974 Mar;112(3):1250–1254. [PubMed] [Google Scholar]
  15. Gregoriades A., Old L. J. Isolation and some characteristics of a group-specific antigen of the murine leukemia viruses. Virology. 1969 Feb;37(2):189–202. doi: 10.1016/0042-6822(69)90198-6. [DOI] [PubMed] [Google Scholar]
  16. Hehlmann R., Schetters H., Erfle V., Leib-Mösch C. Detection and biochemical characterization of antigens in human leukemic sera that cross-react with primate C-type viral proteins (Mr 30,000). Cancer Res. 1983 Jan;43(1):392–399. [PubMed] [Google Scholar]
  17. Hsu S. M., Raine L., Fanger H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol. 1981 May;75(5):734–738. doi: 10.1093/ajcp/75.5.734. [DOI] [PubMed] [Google Scholar]
  18. Imamura M., Phillips P. E., Mellors R. C. The occurrence and frequency of type C virus-like particles in placentas from patients with systemic lupus erythematosus and from normal subjects. Am J Pathol. 1976 May;83(2):383–394. [PMC free article] [PubMed] [Google Scholar]
  19. Kalter S. S., Helmke R. J., Heberling R. L., Panigel M., Fowler A. K., Strickland J. E., Hellman A. Brief communication: C-type particles in normal human placentas. J Natl Cancer Inst. 1973 Apr;50(4):1081–1084. doi: 10.1093/jnci/50.4.1081. [DOI] [PubMed] [Google Scholar]
  20. Kalter S. S., Helmke R. J., Panigel M., Heberling R. L., Felsburg P. J., Axelrod L. R. Observations of apparent C-type particles in baboon (Papio cynocephalus) placentas. Science. 1973 Mar 30;179(4080):1332–1333. doi: 10.1126/science.179.4080.1332. [DOI] [PubMed] [Google Scholar]
  21. Klavins J. V., Shapiro S. H., Wessely Z., Berkman J. I. RNA virus associated antigen in human placenta. Ann Clin Lab Sci. 1980 Mar-Apr;10(2):137–142. [PubMed] [Google Scholar]
  22. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  23. Maeda S., Mellors R. C., Mellors J. W., Jerabek L. B., Zervoudakis I. A. Immunohistologic detection of antigen related to primate type C retrovirus p30 in normal human placentas. Am J Pathol. 1983 Sep;112(3):347–356. [PMC free article] [PubMed] [Google Scholar]
  24. Martin M. A., Bryan T., Rasheed S., Khan A. S. Identification and cloning of endogenous retroviral sequences present in human DNA. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4892–4896. doi: 10.1073/pnas.78.8.4892. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Mellors R. C., Mellors J. W. Antigen related to mammalian type-C RNA viral p30 proteins is located in renal glomeruli in human systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1976 Jan;73(1):233–237. doi: 10.1073/pnas.73.1.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Mellors R. C., Mellors J. W. Type C RNA virus-specific antibody in human systemic lupus erythematosus demonstrated by enzymoimmunoassay. Proc Natl Acad Sci U S A. 1978 May;75(5):2463–2467. doi: 10.1073/pnas.75.5.2463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Nelson J., Leong J. A., Levy J. A. Normal human placentas contain RNA-directed DNA polymerase activity like that in viruses. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6263–6267. doi: 10.1073/pnas.75.12.6263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Nowinski R. C., Old L. J., O'Donnell P. V., Sanders F. K. Serological identification of hamster oncornaviruses. Nat New Biol. 1971 Apr 28;230(17):282–284. doi: 10.1038/newbio230282a0. [DOI] [PubMed] [Google Scholar]
  29. O'Brien S. J., Bonner T. I., Cohen M., O'Connell C., Nash W. G. Mapping of an endogenous retroviral sequence to human chromosome 18. Nature. 1983 May 5;303(5912):74–77. doi: 10.1038/303074a0. [DOI] [PubMed] [Google Scholar]
  30. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  31. Oroszlan S., Copeland T., Summers M. R., Smythers G., Gilden R. V. Amino acid sequence homology of mammalian type C RNA virus major internal proteins. J Biol Chem. 1975 Aug 25;250(16):6232–6239. [PubMed] [Google Scholar]
  32. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415–7419. doi: 10.1073/pnas.77.12.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Rabin H., Benton C. V., Tainsky M. A., Rice N. R., Gilden R. V. Isolation and characterization of an endogenous type C virus of rhesus monkeys. Science. 1979 May 25;204(4395):841–842. doi: 10.1126/science.87013. [DOI] [PubMed] [Google Scholar]
  34. Repaske R., O'Neill R. R., Steele P. E., Martin M. A. Characterization and partial nucleotide sequence of endogenous type C retrovirus segments in human chromosomal DNA. Proc Natl Acad Sci U S A. 1983 Feb;80(3):678–682. doi: 10.1073/pnas.80.3.678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Sawyer M. H., Nachlas N. E., Jr, Panem S. C-type viral antigen expression in human placenta. Nature. 1978 Sep 7;275(5675):62–64. doi: 10.1038/275062a0. [DOI] [PubMed] [Google Scholar]
  36. Schidlovsky G., Ahmed M. C-type virus particles in placentas and fetal tissues of Rhesus monkeys. J Natl Cancer Inst. 1973 Jul;51(1):225–233. doi: 10.1093/jnci/51.1.225. [DOI] [PubMed] [Google Scholar]
  37. Seman G., Levy B. M., Panigel M., Dmochowski L. Type-C virus particles in placenta of the cottontop marmoset (Saguinus oedipus). J Natl Cancer Inst. 1975 Jan;54(1):251–252. doi: 10.1093/jnci/54.1.251. [DOI] [PubMed] [Google Scholar]
  38. Strand M., August J. T. Structural proteins of mammalian RNA tumor viruses: relatedness of the interspecies antigenic determinants of the major internal protein. J Virol. 1975 Jun;15(6):1332–1341. doi: 10.1128/jvi.15.6.1332-1341.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Strand M., August J. T. Type-C RNA virus gene expression in human tissue. J Virol. 1974 Dec;14(6):1584–1596. doi: 10.1128/jvi.14.6.1584-1596.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Suni J., Wahlström T., Vaheri A. Retrovirus p30-related antigen in human syncytiotrophoblasts and IgG antibodies in cord-blood sera. Int J Cancer. 1981 Nov 15;28(5):559–566. doi: 10.1002/ijc.2910280505. [DOI] [PubMed] [Google Scholar]
  41. Todaro G. J., Benveniste R. E., Sherwin S. A., Sherr C. J. MAC-1, a new genetically transmitted type C virus of primates: "low frequency" activation from stumptail monkey cell cultures. Cell. 1978 Apr;13(4):775–782. doi: 10.1016/0092-8674(78)90227-1. [DOI] [PubMed] [Google Scholar]
  42. Todaro G. J., Sherr C. J., Sen A., King N., Daniel M. D., Fleckenstein B. Endogenous New World primate type C viruses isolated from owl monkey (Aotus trivirgatus) kidney cell line. Proc Natl Acad Sci U S A. 1978 Feb;75(2):1004–1008. doi: 10.1073/pnas.75.2.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Vernon M. L., McMahon J. M., Hackett J. J. Additional evidence of type-C particles in human placentas. J Natl Cancer Inst. 1974 Mar;52(3):987–989. doi: 10.1093/jnci/52.3.987. [DOI] [PubMed] [Google Scholar]

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