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. Author manuscript; available in PMC: 2015 Feb 20.
Published in final edited form as: J Mol Biol. 2013 Dec 1;426(4):830–842. doi: 10.1016/j.jmb.2013.11.021

Figure 5. PFKFB3 S-glutathionylation increases the carbohydrate flux to the PPP.

Figure 5

(a) Intracellular Fru-2,6-P2 concentration, (b) the secreted lactate levels (c), cellular concentration of Ribul-5P and Xyl-5P, (d) NADPH, and (e) GSH/GSSG ratio in HeLa cells transiently expressing empty vector pcDNA3.1 (Con), expression plasmids for WT- or C206A-PFKFB3 after treatment with 100 μM H2O2, 50 μM BCNU, or 1.5 mM GSHee to induce PFKFB3 S-glutathionylation. Absolute values were normalized to the total protein concentration and changes in metabolite levels are expressed as a percent of control (without oxidant treatment). T-test (*P < 0.05, **P < 0.01, ***P< 0.001, $P<0.06 (marginally significant); N.S., not significant) used for all the rest figures.