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Growth of LVS ΔfupA/B and LVS ΔfslE ΔfupA/B complements on iron-limiting agar. Tenfold serial dilutions of LVS ΔfupA/B or LVS ΔfslE ΔfupA/B complemented with different genes as indicated were spotted on iron-replete (MHA) or iron-limiting (CDM-Fe) plates. LVS carrying the vector alone was used as control. Growth was recorded after 2 days (MHA) or 4 days (CDM-Fe).
