Fig. 7.
Expression of fslE in bacterial strains. Whole-cell lysates and outer membrane preparations from bacteria as indicated were analysed by Western blotting for expression of FslE and FupA/B. FipB was used as loading control. (a) Lysates of LVS and ΔfslE and ΔfupA/B mutants and corresponding outer membrane proteins (5 µg) were analysed. (b) Lysates of LVS ΔfslE ΔfupA/B complemented with fslE derived from LVS (fslELVS) or from Schu S4 (fslESchu) and corresponding outer membrane proteins (2 µg) were analysed.