Table 2. Twenty shortest cellular chain length mutants.

See Table S1 for complete mutant list.

Mutant	L (µm)*	P-value†	Product	COG	KEGG
Ssx_1116	5.01	3.52e−24	Hypothetical protein
Ssx_1796	5.32	1.69e−24	Transcription elongation factor GreA	Transcription	Transcriptional regulator
Ssx_1103	5.86	5.08e−20	Hypothetical protein
Ssx_1110	6.10	5.97e−18	Hypothetical protein
Ssx_2312	6.12	3.02e−22	Hypothetical protein
Ssx_2147	6.20	1.32e−18	Hypothetical protein
Ssx_0105	6.25	6.54e−14	Uridine kinase	Nucleotide transport and metabolism	Pyrimidine metabolism
Ssx_2296	6.38	1.88e−21	XRE family transcriptional regulator	Transcription	Transcriptional regulator
Ssx_2077	6.40	2.13e−15	Hypothetical protein	General function prediction only
Ssx_2337	6.41	7.03e−19	Hypothetical protein	Function unknown
Ssx_2152	6.43	1.10e−15	ABC-type transporter (uncharacterized), ATPase component	General function prediction only	ABC transporter
Ssx_2333	6.51	9.01e−17	Integral membrane protein	Cell envelope biogenesis, outer membrane
Ssx_2250	6.52	8.40e−18	Peptide ABC transporter permease	Function unknown
Ssx_0773	6.64	9.18e−15	PTS enzyme I	Carbohydrate transport and metabolism	Phosphotransferase system (PTS)
Ssx_2332	6.65	1.54e−18	d-Alanine-poly(phosphoribitol) ligase subunit 2	Lipid metabolism/secondary metabolites biosynthesis, transport and catabolism	d-Alanine metabolism
Ssx_1860	6.69	3.60e−16	Penicillin-binding protein 1A	Cell envelope biogenesis, outer membrane	Peptidoglycan biosynthesis
Ssx_2285	6.77	1.40e−15	Hypothetical protein
Ssx_2162	6.79	1.17e−12	Hypothetical protein
Ssx_0148	6.83	7.10e−14	Sugar ABC transporter, ATP-binding protein	Carbohydrate transport and metabolism	ABC transporter
Ssx_2141	6.83	3.19e−14	Argininosuccinate lyase	Amino acid transport and metabolism	Alanine, aspartate and glutamate metabolism

^*L, Cellular chain length.

^†P-value for statistically significant difference compared with SK36.