Figure 6. The development of RORγt⁺ T helper cells, but not RORγt⁺ ILCs is dependent on STAT3 signaling.

(A–F) Colonic LPLs were isolated from Stat3^fl/fl and Rorc-cre-Stat3^fl/fl littermate mice before or 5 days after C. rodentium infection.

(A, D) RORγt and CD4 expression were analyzed in colonic LPLs from naïve mice by flow cytometry after gating on Thy1⁺ CD3⁺ adaptive lymphocytes and Thy1⁺ CD3⁻ innate lymphoid cells.

(B, C) Percentage of RORγt⁺ cells in the CD4⁺ CD3⁺ T cell population, as well as the absolute numbers of RORγt⁺ CD4⁺ T cells in the colons of naïve and C. rodentium infected Stat3^fl/fl and Rorc-cre-Stat3^fl/fl mice are shown.

(E, F) Percentage of RORγt⁺ cells in the CD3⁻ Thy1⁺ ILCs population, as well as the absolute numbers of RORγt⁺ ILCs in the colons of naïve and C. rodentium infected Stat3^fl/fl and Rorc-cre-Stat3^fl/fl mice are shown.

**P<0.01, ***P<0.001; ns, no significant difference (Student’s t-test). Data are representative of three independent experiments (mean ± s.e.m.). Each dot represents one individual mouse (B, C, E, F). See also Figure S6.