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Schematic representation of RNA–DNA hybrid re-association and release of multiple functionalities: FRET response, DS siRNA (in red) and MG RNA aptamer (in green). Three-dimensional (3D) structure of the two-stranded MG aptamer (in green) contains a bound dye (in red). PDB ID: 1f1t. Due to asymmetry of the MG aptamer, the resulting DNA duplex is also asymmetric and contains an internal loop.
