Figure 4.

Release of multiple DS siRNA targeting upon re-association of RNA–DNA hybrids. (a) Schematic of hybrid re-association and native PAGE demonstrating the release of three and seven DS siRNA. (b and c) IFN activity assessed using THP-1 IFN reporter cells that secrete alkaline phosphatase in response to type I IFN. Cells were transfected with hybrids, and cell culture supernatants were assayed for reporter activity after 24 h. (b) Cells were transfected with hybrids designed to release varying numbers of DS. (c) THP-1 and THP-1 deficient for STING (in gray) were transfected with hybrids releasing seven DS siRNAs.