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. 2013 Nov 13;42(3):2003–2014. doi: 10.1093/nar/gkt1071

Figure 2.

Figure 2.

Small-sized sodF RNA that contains the 19 nt sodN-complementary (anti-sodN) sequence is produced in a Nur-dependent manner. (A) S1 mapping of sodF RNAs with DNA probe 5′-end labeled at +60 position relative to the stop codon of the sodF ORF. RNAs were prepared from the wild-type (M145) and △nur mutant grown in the presence and absence of 50 μM NiSO4. The arrows indicate the presence of sodF RNAs that fully protected the probe (sodF) and partly protected (s-SodF). FP denotes free probe with plasmid vector sequence attached. (B) Northern blot analysis of sodF RNAs. RNAs were prepared from the wild type (M145), △sodF, △sodF2 and △nur cells grown in the presence and absence of 50 μM NiSO4. RNAs were run on 12.5% PAG to resolve small-sized RNAs and hybridized with 22 nt single-stranded DNA probe (+18 to +39 nt relative to the sodF stop codon) labeled at 5′-end by 32P (Figure 1). The 5S rRNA band electrophoresed on agarose gel was shown in parallel to demonstrate the quantity and quality of RNA samples.