Figure 4.

Sensitivity of s-SodF RNA toward 5′ monophosphate-dependent exonuclease. The phosphorylation state of the s-SodF RNA was monitored by treatment with a 5-monophosphate-dependent exonuclease (5′-Exo). Total RNA isolated from wild type (M145) and △nur cells were subjected to digestion with 5′-Exo and analyzed by gel electrophoresis (lower panel) and northern blotting with an s-SodF specific radiolabeled probe (upper panel). RNA samples were treated either with or without TAP that converts 5′-triphosphorylated RNA to 5′-monophosphorylated one prior to 5′-Exo treatment. The status of stable RNAs in each sample is shown in the lower panel.