Figure 5.

Effect of s-SodF RNA on the stability of sodN mRNA. (A) S1 mapping of sodF and sodN RNAs following rifampicin treatment. Wild-type (M145) and △sodF cells with or without pSET-based recombinant plasmid expressing s-SodF RNA from ermE* promoter (p-s-SodF) in the chromosome were grown in YEME medium to OD600 of 0.8 and treated with rifampicin (300 μg/ml). At 5, 10, 15 and 20 min after rifampicin treatment, cells were harvested and fixed with methanol. RNA samples were analyzed by S1 mapping with 5-end-labeled probes. A representative result was presented. (B) The relative amount of each RNA species was plotted to estimate half-life.