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. 2013 Nov 11;42(3):1970–1986. doi: 10.1093/nar/gkt1118

Figure 2.

Figure 2.

Endogenous HOW binds dgrasp mRNA in Drosophila ovaries. (A) Agarose gel electrophoresis of RT-PCR reactions from RIP from WT Drosophila ovaries using anti-HOW (HOW RIP) or IgG (IgG RIP). Transcripts scored in the RIP reactions are dgrasp and Neurexin IV mRNAs; shotgun was used as a negative control. (B) Relative enrichment of endogenous dgrasp, Neurexin IV and shotgun in HOW RIP compared with IgG RIP measured by real-time RT–qPCR. Values are means ± standard deviations (SD) (error bars) (n = 3). (C) Agarose electrophoresis of PCR product from endogenous dgrasp and Neurexin IV mRNA immunoprecipitated from Drosophila ovaries overexpressing HOW(S)-HA and HOW(L)-HA using an anti-HA antibody [HOW(L)-HA RIP and HOW(S)-HA RIP] or an IgG as control (IgG RIP). Note that dgrasp mRNA binds HOW(S)-HA. (D) Relative enrichment of dgrasp and Neurexin IV in HOW(S)-HA and HOW(L)-HA RIP when compared with IgG RIP measured by real-time RT–qPCR. Values are means ± SD (error bars) (n = 3).