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. 2013 Nov 11;42(3):1970–1986. doi: 10.1093/nar/gkt1118

Figure 6.

Figure 6.

HRE1 is sufficient to mediate dgrasp RNA stability but not targeting to the open ZOC. (A) Time course degradation assay of full-length dgrasp and dgrasp-ΔHRE1 RNAs measured by real-time RT–qPCR. Total RNA was isolated at the indicated times (0, 45 and 180 min). The values shown are averages ± SD of three independent experiments performed in duplicate. (B–C′) Fluorescent in situ hybridization of CT-dgrasp::gfp (B) and CT2-dgrasp::gfp (C) fragments using anti-sense GFP RNA probe (red) in the follicular epithelium of stage 10B transgenic ovaries overexpressing either of the two dgrasp mRNA fragments. Plasma membrane is outlined by immunolabelling with α-spectrin (green). B′ and C′ are insets of C and D, respectively. Note CT2-dgrasp::gfp fragment is expressed in the follicular epithelium, but does not localize to the open ZOC. Scale bars: 10 µm.