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Overall structure of the RluB-RNA complex. (A) Structure of RluB-bound to a small RNA substrate. The S4 domain is blue, the catalytic domain is blue–green and the RNA phosphate backbone is gold. Nucleosides are shown as gold sticks. The catalytic aspartate 110 and tyrosine 140 are shown in ball-and-stick form with side chains colored gold. (B) Schematic drawing of the 21-mer rRNA small substrate of RluB. For crystallization U2605 was substituted by 5-FU.
