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. 2013 Nov 5;42(3):1711–1720. doi: 10.1093/nar/gkt1040

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Extension of the HLTF-mediated D-loops. (A) Comparison of level of HLTF- or yRad51/yRad54-mediated D-loop formation used for extension reaction in (B). A radioactively labelled (panels A and C) or unlabelled (panel B) D1 oligonucleotide was used for the D-loop assay. (B) D-loop generated with unlabelled D1 oligonucleotide was extended by yeast DNA polymerase δ. Extension of the D-loop is monitored by incorporation of ³²P labelled dATP. (C) Radioactively labelled D-loop was used for the D-loop reaction followed by the extension with DNA polymerase δ. Reactions were stopped by addition of SDS/proteinase K and reaction products were resolved on 0.8% native agarose gel. The positions of the free oligonucleotide, D-loop and extended D-loop are indicated together with the quantification of D-loop (as a percentage of D-loop formed versus free oligonucleotide) and their extension products (as a percentage of D-loop extended versus not extended).