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. 2013 Nov 8;42(3):2049–2063. doi: 10.1093/nar/gkt1107

Figure 3.

Figure 3.

rpL10 loop mutants alter the rotational equilibrium of the ribosome. (a) The B7a intersubunit bridge. In the non-rotated state, A2207 (25S rRNA) forms an interaction with G913 (18S rRNA). In the rotated state, that interaction is disrupted, and the marked 2′ OH-group on A2207 becomes accessible to modification by 1M7. Similarly, marked atoms on G913 become accessible to modification by kethoxal upon rotation. (b) Reactivity peaks obtained by hSHAPE after probing of the landmark base A2207 (arrows) at the LSU side of the B7a intersubunit bridge with 1M7. (c) Reactivity peaks obtained by hSHAPE after chemical probing of the landmark base G913 at the SSU side of the B7a intersubunit bridge with kethoxal. Data were assigned five levels of reactivity: 0 (for less than a trace’s median value), 1 (for a value between the median and the mean), 2 (for a value between the mean and the first standard deviation), 3 (for a value between the first and the second standard deviation) and 4 (for values above the second standard deviation). Mean, median and standard deviation were calculated on a per trace basis.