Figure 4.

rpL10 loop mutants affect peptidyltransferase activity and translational fidelity. (a) Apparent rates of peptidyltransfer from single turnover peptidylpuromycin reactions for indicated ribosomes. (b) Programmed −1 and +1 ribosomal frameshifting values obtained using dual-luciferase reporters. (c) Misincorporation of a stop codon, and near- and non-cognate amino acids in mutants compared to wild-type levels as monitored using dual-luciferase reporters. Bars indicate SEM (n = 4), *P < 0.05, **P < 0.01.