Table 1.
Residues E72 in Snu13p and residues W245 and R249 in Rsa1p are required for optimal yeast cell growth
| (A) | ||
|---|---|---|
| Strain Gal::SNU13 | G (h) | Growth defect (% WT) |
| pG1::SNU13-WT | 3.26±0.04 | |
| pG1::SNU13-E72D73K74/AAA | 3.56±0.05 | –9.2 |
| pG1::SNU13-E72/A | 3.58±0.09 | –9.8 |
| pG1::SNU13-L69/A | 3.22±0.09 | +1.2 |
| (B) | ||
|---|---|---|
| Strain ΔRSA1 | G (h) | Growth defect (% WT) |
| pG1::(-) | 3.74±0.18 | –44.2 |
| pG1::RSA1-WT | 2.6±0.13 | |
| pG1::RSA1-W245/A | 3.06±0.10 | –17.8 |
| pG1::RSA1-R246/A | 2.55±0.10 | +1.7 |
| pG1::RSA1-R249/A | 3.24±0.03 | –24.8 |
(A) Effects of ectopic expression of WT or variant Snu13p proteins from recombinant pG1::SNU13 plasmid on growth of YPH499-GAL::SNU13 yeast cell. YPH499-GAL::SNU13 cells were transformed with recombinant pG1::SNU13 plasmids expressing WT or mutated Snu13p. Transformed cells were grown at 30°C in YPD medium containing glucose in order to block genomic expression of Snu13p. Cells were maintained in exponential growth by successive dilutions. The effects of the ectopic expression of WT and mutated Snu13p on cell growth were monitored by measuring the absorbance at 600 nm. The cell doubling times (G) were calculated and are expressed in hours (second column). Estimated errors on the G-values were calculated from three distinct experiments performed in the same conditions. G-values were used to calculate a growth defect percentage for each variant Snu13p protein (third column). (B) Effects of the ectopic expressions of WT and variant Rsa1p from recombinant pG1::RSA1 plasmid on growth of an RSA1 KO strain. Same legend as in panel A.