Figure 3.

Histopathologic features of experimental alcohol-induced steatohepatitis-effects of myriocin treatment. Adult male Long Evans rats were fed with isocaloric liquid diets containing 0% (control) or 37% ethanol (caloric content) for 8 weeks, and treated with vehicle or myriocin three times per week over the last 5 weeks of the experiment. Formalin-fixed paraffin-embedded 5 μm thick sections of liver were stained with H&E. (a) Control livers had regular chord-like architectures and uniform hepatocyte structure. (b) Myriocin treatment had no discernible effect on hepatic architecture in control rats. (c) Ethanol-exposed livers exhibited loss of the chord-like architecture with marked variability in hepatocyte cytology, prominent micro-vesicular steatosis (clear cytoplasmic vacuoles), foci of lymphomononuclear cell inflammation, and apoptotic bodies (arrows). (d) Myriocin treatment nearly normalized hepatic architecture in ethanol-fed rats.