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. 2014 Feb 7;20:163–170.

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Copyright © 2014 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Validation of pressure transduction accuracy. A pressure transducer (PT#1) was connected to a 35-G needle that was inserted into the lumen of a closed hub of a rigid three-way connector directly attached to a second pressure transducer (PT#2). At flow rates of 0.30±0.012 (mean ± standard deviation [SD]) and 0.52±0.0002 µl/min, the pressure difference between PT#1 and PT#2 was 0.012±0.0086 and 0.13±0.029 mmHg, respectively. This represented a small artifactual increase in transduced pressure in PT#1 due to needle resistance. Thus, at an outflow rate of 0.086 µl/min corresponding to physiologic mouse intraocular pressure (IOP) of 15 mmHg, PT#1 transduced pressure marginally overestimated IOP (PT#2) by a mean of 0.059 mmHg or 0.02%. This marginal difference was considered negligible.