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. 2014 Feb 10;9(2):e87230. doi: 10.1371/journal.pone.0087230

Figure 5. Western blot analyses of insulin stimulated and basal tissue.

Figure 5

Protein phosphorylation analyses of Akt and AMPK levels were performed on protein extracted from insulin stimulated (a) gonadal adipose tissue, (b) gastrocnemius muscle, (c) liver in insulin stimulated liver of 6 month-old male wildtype (WT) and aromatase knockout (KO) and 2.5 µg/day estrogen-replaced KO (KOE) mice. Expression data from 6–8 samples per genotype are shown, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus expression in age-matched WT samples and #p<0.05, ###p<0.001 versus KO samples.