Table 1. Real-time PCR primers.
Transcript | Oligonucleotide pairs (5′-3′) | Amplicon Size (bp) |
Glucose-6-phosphatase (G6Pase) | For: TCTTGTGGTTGGGATACTGG | 110 |
Rev: AGCAATGCCTGACAAGACTC | ||
Phosphophenolpyruvate carboxykinase (PEPCK) | For: GTGTCCCCCTTGTCTATGAA | 163 |
Rev: GGTATTTGCCGAAGTTGTAG | ||
Insulin Receptor Substrate 1 (IRS1) | For;CAGCGATGGCGAAGGCACCA | 272 |
Rev;GCGAACCGGACACGGAAGCA | ||
Insulin Receptor Substrate 2 (IRS2) | For: GCGGCCTCATCTTCTTCACT | 129 |
Rev: AACTGAAGTCCAGGTTCATATA | ||
Glycogen Substrate Kinase 3 beta (GSK3β) | For: ACCAATATTTCCTGGGGACA | 130 |
Rev: GTACCTTGATTTGAGGGAAT | ||
Fatty Acid Synthase (Fasn) | For: CACAGATGATGACAGGAGATGGA | 205 |
Rev: TCGGAGTGAGGCTGGGTTGATA | ||
Stearoyl CoA desaturase 1 (Scd-1) | For: CATCATTCTCATGGTCCTGCT | 236 |
Rev: CCCAGTCGTACACGTCATT | ||
Acetyl-CoA Carboxylas (ACCα) | For: TGTTGGGGTTATTTCAGTGTTGC | 236 |
Rev: TGTCCAGCCAGCCAG GTCG | ||
Cyclophilin | For: CTTGGGCCGCGTCTCCTTC | 180 |
Rev: TGCCGCCAGTGCCATTAT |
List of primer sequences (and expected amplicon sizes) used for Real-Time PCR.