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. 2014 Feb 10;9(2):e87230. doi: 10.1371/journal.pone.0087230

Table 1. Real-time PCR primers.

Transcript Oligonucleotide pairs (5′-3′) Amplicon Size (bp)
Glucose-6-phosphatase (G6Pase) For: TCTTGTGGTTGGGATACTGG 110
Rev: AGCAATGCCTGACAAGACTC
Phosphophenolpyruvate carboxykinase (PEPCK) For: GTGTCCCCCTTGTCTATGAA 163
Rev: GGTATTTGCCGAAGTTGTAG
Insulin Receptor Substrate 1 (IRS1) For;CAGCGATGGCGAAGGCACCA 272
Rev;GCGAACCGGACACGGAAGCA
Insulin Receptor Substrate 2 (IRS2) For: GCGGCCTCATCTTCTTCACT 129
Rev: AACTGAAGTCCAGGTTCATATA
Glycogen Substrate Kinase 3 beta (GSK3β) For: ACCAATATTTCCTGGGGACA 130
Rev: GTACCTTGATTTGAGGGAAT
Fatty Acid Synthase (Fasn) For: CACAGATGATGACAGGAGATGGA 205
Rev: TCGGAGTGAGGCTGGGTTGATA
Stearoyl CoA desaturase 1 (Scd-1) For: CATCATTCTCATGGTCCTGCT 236
Rev: CCCAGTCGTACACGTCATT
Acetyl-CoA Carboxylas (ACCα) For: TGTTGGGGTTATTTCAGTGTTGC 236
Rev: TGTCCAGCCAGCCAG GTCG
Cyclophilin For: CTTGGGCCGCGTCTCCTTC 180
Rev: TGCCGCCAGTGCCATTAT

List of primer sequences (and expected amplicon sizes) used for Real-Time PCR.