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. 2014 Feb 10;9(2):e87210. doi: 10.1371/journal.pone.0087210

Figure 5. Absence of mAb 8B6-specific OAcGD2 in mouse neuroblastoma sera.

Figure 5

I-TLC of gangliosides from rat brain (Lane 0), NXS2 neuroblastoma tumor bearing mice sera (Lane 2), and mouse control sera (Lane 1) stained with resorcinol-HCl (Panel A) or immunostained with chimeric mAbs c.8B6 (Panel B), or ch14.18 (Panel C). Total ganglioside fractions presented in lanes 2 and 3 represents 1 ml of serum. Ganglioside GD2 was detected in the neuroblastoma-tumor bearing mice but not in the control sera when mAb ch14.18 was used. In contrast, no OAcGD2 was detected in sera from neuroblastoma-tumor bearing mice and control mouse sera when either mAb c.8B6 or ch14.18 was tested. Serums from 6 different mice were tested with the same results.