. 2014 Feb 10;9(2):e87208. doi: 10.1371/journal.pone.0087208

Table 1. EN2 levels comparisons between affection and genotype groups.

Genotype	Affection	Least squared mean^a	Standard errors^b	P-value^c	Fold change^d	Standard errors^e
AC/AC	Autism vs. Control	0.007615	0.1462	0.9586	0.9947	0.1015
AC/GT		−0.7963	0.2188	0.0005	1.7366	0.2678
GT/GT		0.2897	0.1599	0.0744	0.8180	0.0915
Affection	Genotype	Least squared mean^a	Standard errors^b	P-value^c	Fold change^d	Standard errors^e
Autism	AC/AC vs. AC/GT	0.6236	0.2273	0.0077	0.6490	0.1041
	AC/AC vs. GT/GT	0.09040	0.1940	0.6426	0.9393	0.1280
	AC/GT vs. GT/GT	−0.5332	0.2315	0.0243	1.4471	0.2366
Control	AC/AC vs. AC/GT	−0.1803	0.1300	0.1699	1.1331	0.1027
	AC/AC vs. GT/GT	0.3725	0.08992	<.0001	0.7724	0.0483
	AC/GT vs. GT/GT	0.5527	0.1373	0.0001	0.6817	0.0653

Least squared means were calculated from ΔΔCt values (ΔCt^autism–ΔCt^control or ΔCt^{1st genotype}–ΔCt^{2nd genotype}) after adjusting for significant covariates.

Standard error for least squared mean.

Type 3 tests of fixed effects were performed considering all the significant covariates. Significant values (5% cut-off) are in bold.

Fold change of each first group versus the second is calculated as 2^{−(least squared mean)}.

Standard error of fold change.