Table 1. EN2 levels comparisons between affection and genotype groups.
Genotype | Affection | Least squared meana | Standard errorsb | P-valuec | Fold changed | Standard errorse |
AC/AC | Autism vs. Control | 0.007615 | 0.1462 | 0.9586 | 0.9947 | 0.1015 |
AC/GT | −0.7963 | 0.2188 | 0.0005 | 1.7366 | 0.2678 | |
GT/GT | 0.2897 | 0.1599 | 0.0744 | 0.8180 | 0.0915 | |
Affection | Genotype | Least squared meana | Standard errorsb | P-valuec | Fold changed | Standard errorse |
Autism | AC/AC vs. AC/GT | 0.6236 | 0.2273 | 0.0077 | 0.6490 | 0.1041 |
AC/AC vs. GT/GT | 0.09040 | 0.1940 | 0.6426 | 0.9393 | 0.1280 | |
AC/GT vs. GT/GT | −0.5332 | 0.2315 | 0.0243 | 1.4471 | 0.2366 | |
Control | AC/AC vs. AC/GT | −0.1803 | 0.1300 | 0.1699 | 1.1331 | 0.1027 |
AC/AC vs. GT/GT | 0.3725 | 0.08992 | <.0001 | 0.7724 | 0.0483 | |
AC/GT vs. GT/GT | 0.5527 | 0.1373 | 0.0001 | 0.6817 | 0.0653 |
Least squared means were calculated from ΔΔCt values (ΔCtautism–ΔCtcontrol or ΔCt1st genotype–ΔCt2nd genotype) after adjusting for significant covariates.
Standard error for least squared mean.
Type 3 tests of fixed effects were performed considering all the significant covariates. Significant values (5% cut-off) are in bold.
Fold change of each first group versus the second is calculated as 2−(least squared mean).
Standard error of fold change.