. 2014 Feb 10;9(2):e88328. doi: 10.1371/journal.pone.0088328

Table 1. M. bovis proteins identified by mass spectrometry and reactions with sera from naturally infected cattle in immunoblotting experiments.

Spot no.	Protein	NCBI ID	pI	MW (kDa)	Identificationscores	Protein score C.I. %^a	COG^b
1	Peptide chain release factor 1	gi\|313678884	5.30	40.43	842	100	J
2	Pyrimidine-nucleoside phosphorylase	gi\|313678400	8.09	47.26	1020	100	F
3	Glyceraldehyde-3-phosphate dehydrogenase, type 1	gi\|313678191	7.70	36.85	814	100	G
4	Pyruvate dehydrogenase E1 component subunit alpha	gi\|313678230	6.13	41.33	435	100	C
5	L-lactate dehydrogenase	gi\|313678425	6.35	35.02	962	100	C
6	Oxidoreductase, zinc-binding dehydrogenase family	gi\|313678628	6.51	37.96	778	100	E
7	Acetate kinase	gi\|339320670	6.16	44.27	889	100	C
8	D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase	gi\|313678257	6.30	89.91	929	100	G
9	Pyruvate dehydrogenase complex, E3 component, dihydrolipoamide dehydrogenase	gi\|313678233	6.50	58.80	569	100	C
10	Adenylate kinase	gi\|344204920	8.53	24.33	76	99.91	F
11	Thymidine phosphorylase(TDRPASE)	gi\|339321078	7.60	47.28	995	100	F
12	Translation elongation factor P	gi\|313678493	5.36	21.00	148	100	J
13	Cell division protein ftsZ	gi\|339320924	4.86	41.95	110	100	D
14	Thioredoxin-disulfide reductase	gi\|313678228	5.50	33.89	164	100	O
15	Pyruvate dehydrogenase E1 component subunit beta	gi\|313678231	5.44	36.18	1070	100	C
16	Phosphopentomutase	gi\|313678465	5.32	44.02	854	100	G
17	Lipoyltransferase and lipoate-protein ligase	gi\|313678142	5.43	39.78	693	100	H
18	Translation elongation factor Ts	gi\|313678643	5.23	32.67	438	100	J
19	Purine nucleoside phosphorylase	gi\|313678401	5.33	25.92	956	100	F

Protein spots from 2-DE were sequenced using MALDI-TOF/TOF MS and identified by searching mycoplasma databases using the MASCOT search engine 2.2. A GPS explorer protein confidence index ≥95% were used for further manual validation.

C.I. %: the confidence interval for the protein score.

COG database functional classes: (C) energy production and conversion, (D) cell cycle control, cell division, chromosome partitioning, (E) amino acid transport and metabolism, (F) nucleotide transport and metabolism, (G) carbohydrate transport and metabolism, (H) coenzyme transport and metabolism, (J) translation, ribosomal structure and biogenesis, (O) post-translational modification, protein turnover, chaperones.