Skip to main content
. 2014 Feb 10;9(2):e88541. doi: 10.1371/journal.pone.0088541

Figure 6. Activity of trypsin under chemical stress following incubation with chemical chaperones of different chemical families.

Figure 6

The activity of trypsin under chemical stress, using 30% TFE when incubated with: (A) methylamines, (B) amino acids, (C) sugars and (D) polyols. Trypsin, 125 µg/ml was inactivated by the addition of 30% TFE. Trypsin was incubated in the presence or in the absence of chemical chaperones. After adding BAPNA as a substrate, absorbance signal at 414 nm was recorded every 30 seconds for five minutes and trypsin activity was derived from the slope of the measurements. 100% activity represents non-heated trypsin.