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. 2014 Feb 10;9(2):e88723. doi: 10.1371/journal.pone.0088723

Figure 4. CuO NP behavior in experimental tanks.

Figure 4

(A) CuO NP concentration during exposure in water samples from the experimental tanks (showed mean ± SD). (B) Time course of CuO NP concentration (mean % ± SD) relative to T = 0 for in FW0.5 and FW5 (left), SW0.5 and SW5 (right) treatment groups with fish (+F, black circles) and without fish (−F, open circles). Salinity (in ppt) at each time point is depicted for SW treatments. (C) CuO NP size in experimental FW (left) and SW (right) tanks analyzed by DLS. The inset depicts an example of the log-normal distribution data for the FW0.5 tank at days 0 and 6. Linear trend lines for each treatment are shown. Additionally, tank salinity in relation to time of exposure is shown (light gray lines). FW0, fresh water; FW0.5, FW plus 0.5 mg·L−1 CuO; FW5, FW plus 5 mg·L−1 CuO; SW0, increasing salinity; SW0.5, SW plus 0.5 mg·L−1 CuO; SW5, SW plus 5 mg·L−1 CuO. T = 0 corresponds to the sample taken immediately after CuO NP addition and the initial salinity increase. T = #: sample taken after # days of CuO exposure.