Figure 2. Acacetin reduces intrasynaptosomal Ca²⁺ levels but does not alter the synaptosomal membrane potential and Na⁺ influx.

Cytosolic free Ca²⁺ concentration (nM) (A) or synaptosomal membrane potential (B) was measured in the absence (control) and in the presence of 30 µM acacetin, added 10 min before depolarization with 1 mM 4-AP. C: Na⁺ influx was induced by 1 mM 4-AP in the absence (control) or presence of 30 µM acacetin or 2 µM TTX, added 10 min before depolarization. Results are mean ± SEM of independent experiments, using synaptosomal preparations from five animals. ***, P<0.001 versus control group.