Figure 3. Acacetin-mediated inhibition of glutamate release is prevented by blocking the Ca_v2.2 (N-type) and Ca_v2.1 (P/Q-type) channels.

A: Glutamate release was evoked by 1 mM 4-AP in the absence (control) or presence of 30 µM acacetin, 2 µM ω-CgTX MVIIC, 2 µM ω-CgTX MVIIC and 30 µM acacetin. B: Quantitative comparison of the extent of glutamate release by 1 mM 4-AP in the absence or presence of 30 µM acacetin, and absence and presence of 2 µM ω-CgTX MVIIC, 100 µM dantrolene, or 100 µM CGP37157. Results are mean ± SEM of independent experiments, using synaptosomal preparations from five to six animals. ***, P<0.001 versus control group; #, P<0.05 versus dantrolene-, or CGP37157-treated group.