Figure 3. The PilF protein of V. cholerae might act as pilotin. In our previous study, the localization of the VC1612 protein was not unambiguously predictable and not experimentally addressed.¹⁷ However, BLAST comparisons with PilF of P. aeruginosa indicate that the VC1612 protein of V. cholerae (suggested annotation: PilF) might act as a pilotin, thereby assisting the PilQ secretin in its assembly.