Figure 6.

Changes in the expression of apoptosis-regulating proteins following the knockdown of GRP78 with shRNA. (A) Protein levels of GRP78, CHOP, Bcl-2 and Bax following knockdown of GRP78 in hypopharyngeal carcinoma cells were measured by immunoblot analysis under normoxic and severely hypoxic cultures for 24 h. (B) Histogram of the protein expression levels of GRP78, CHOP, Bcl-2 and Bax in GRP78(+/+) and GRP78(−/−) cells under normoxia and severe hypoxia for 24 h. ^**P<0.01, vs. N24h GRP78(+/+); ^#P<0.05 and ^##P<0.01, vs. N24h GRP78(+/+); ^ΔP<0.05 and ^ΔΔP<0.01, vs. H24h GRP78(+/+). Data shown are from three individual experiments. GRP78, glucose-regulated protein 78; CHOP, C/EBP homology protein; shRNA, short hairpin RNA; N24h, normoxia; H24h, severe hypoxia.