Figure 4.

Resveratrol (Res) regulates the inflammatory response in rabbit articular chondrocytes. (A) Rabbit articular chondrocytes were untreated or treated with 20 μM resveratrol for the indicated time periods. (B) Rabbit articular chondrocytes were untreated or treated with the indicated concentrations of resveratrol for 3 h. PGE₂ production was measured using a PGE₂ assay kit. (C) Rabbit articular chondrocytes or (D) cartilage explants were untreated or treated with 20 μM resveratrol for 24 h. Expression of COX-2 was detected by immunofluorescence staining. The data represent a typical experiment, whereby similar results were obtained from three experiments. ^*P<0.05, compared with untreated cells. PGE₂, prostaglandin E₂.