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. 2013 Dec 24;7(3):635–640. doi: 10.3892/ol.2013.1773

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Changes in autophagy signaling molecules in human gastric adenocarcinoma. (A) A representative image showing protein expression of autophagic signaling molecules in human gastric adenocarcinoma using immunoblotting. Proteins were separated by SDS-PAGE and analyzed by immunoblotting with anti-Beclin 1, anti-P62 and anti-LC3 antibodies. (B) Quantitative analysis of protein levels was performed by densitometric analysis of the bands. Immunoblotting with an anti-actin antibody showed equal amounts of loaded protein in each lane. Data are expressed as percentages of control values (mean ± standard deviation; n=5). ^**P<0.01, vs. control. Con, control; GC, gastric carcinoma; LC3, light chain 3.