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Immunohistochemical localization of Beclin 1 and Bcl-2 in human gastric adenocarcinoma. (A) Double immunostaining with anti-Bcl-2 (green fluorescence) and anti-Beclin 1 (red fluorescence) antibodies showed that (f) increased Beclin 1 expression was associated with (e) Bcl-2 activation in human gastric adenocarcinoma (scale bar, 20 μm). (B) The colocalization of Beclin 1 and Bcl-2 was demonstrated by XZ reconstructions. Con, control; GC, gastric carcinoma; Bcl-2, B-cell lymphoma 2.
