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. 2014 Jan 15;7(3):764–770. doi: 10.3892/ol.2014.1803

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Pathological observation and immunohistochemistry in the control group (C), and hyperthermia groups 1 (H1) and 2 (H2). In the control group, both sides of the tumor tissue contained typical tumor cells (A1, B1, both unheated); in the H1 group, the tumor cells exposed to heat exhibited large areas of necrosis and karyorrhexis (C1, heated side; D1, unheated side); in the H2 group, tumor cells showed a large area of necrosis. The unheated side showed more necrosis than the heated side (E1, heated side; F1, unheated side). Compared with groups C, M and T, the PCNA index was significantly decreased in the H1 group (C2, heated side; D2, unheated side) and the H2 group (E2, heated side; F2, unheated side) (P<0.05). Compared with the H1 group, the PCNA index in the H2 group was significantly decreased (P<0.01), but there was no significant difference in the PCNA indexes between groups C, M and T (P>0.05). PCNA, proliferating cell nuclear antigen.