Table I.
Gene name | Sequence |
---|---|
Bax | Forward: 5′-AAG CTG AGC GAG TGT CTC CGG CG-3 Reverse: 5′-CAG ATG CCG GTT CAG GTA CTC AGT C-3′ |
Bcl-2 | Forward: 5′-CTC GTC GCT ACC GTC GTG ACT TGG-3′ Reverse: 5′-CAG ATG CCG GTT CAG GTA CTC AGT C-3′ |
Caspase-9 | Forward: 5′-GGC CCT TCC TCG CTT CAT CTC-3′ Reverse: 5′-GGT CCT TGG GCC TTC CTG GTA T-3′ |
Caspase-3 | Forward: 5′-CAA ACT TTT TCA GAG GGG ATC G-3′ Reverse: 5′-GCA TAC TGT TTC AGC ATG GCA-3′ |
NF-κB | Forward: 5′-CAC TTA TGG ACA ACT ATG AGG TCT CTG G-3′ Reverse: 5′-CTG TCT TGT GGA CAA CGC AGT GGA ATT TTA GG-3′ |
IκB-α | Forward: 5′-GCT GAA GAA GGA GCG GCT ACT-3′ Reverse: 5′-TCG TAC TCC TCG TCT TTC ATG GA-3′ |
iNOS | Forward: 5′-AGA GAG ATC GGG TTC ACA-3′ Reverse: 5′-CAC AGA ACT GAG GGT ACA-3′ |
COX-2 | Forward: 5′-TTA AAA TGA GAT TGT CCG AA-3′ Reverse: 5′-AGA TCA CCT CTG CCT GAG TA-3′ |
MMP-2 | Forward: 5′-CTT CTT CAA GGA CCG GTT CA-3′ Reverse: 5′-GCT GGC TGA GTA CCA GTA-3′ |
MMP-9 | Forward: 5′-TGG GCT ACG TGA CCT ATG AC-3′ Reverse: 5′-GCC CAG CCC ACC TCC ACT CC-3′ |
TIMP-1 | Forward: 5′-GTC AGT GAG AAG CAA GTC GA-3′ Reverse: 5′-ATG TTC TTC TCT GTG ACC CA-3′ |
TIMP-2 | Forward: 5′-TGG GGA CAC CAG AAG TCA AC-3′ Reverse: 5′-TTT TCA GAG CCT TGG AGG AG-3′ |
GAPDH | Forward: 5′-CGG AGT CAA CGG ATT TGG TC-3′ Reverse: 5′-AGC CTT CTC CAT GGT CGT GA-3′ |
RT-PCR, reverse transcription-polymerase chain reaction.