Table I.
Primer | Primer sequence, 5′-3′ | Optimal annealing temperature, °C |
---|---|---|
FAP ribozyme | ||
2F | CTGCAGGTGGATCTCCTGGTCTTTGTTTCAATACTGATGAGTCCGTGAGGA | 55 |
2R | ACTAGTAAATTAGCATATGTCTATCAAAACAATATTTCGTCCTCAGGACT | 55 |
FAP | ||
F1 | TCCCTTGCTAATTCAAGTGT | 55 |
R1 | AGAGCTTTAGCAATCTGTGC | 55 |
F2 | TGGAAAATGATTTGAAAAAT | 55 |
R2 | CTGTGTAGACAGACGCGTAA | 55 |
GAPDH | ||
F8 | GGCTGCTTTTAACTCTGGTA | 55 |
R8 | GACTGTGGTCATGAGTCCTT | 55 |
FAP, fibroblast activation protein.