Fig. 4.

Effects of EtOH on single BK channel activity in excised patches from native DSM cells under low intracellular [Ca²⁺]. A: an example of current-voltage relationship before and after the addition of 0.3% EtOH measured in the same excised patch. The represented slope single BK channel conductance values were 189 pS for pre-EtOH control and 181 pS for 0.3% EtOH. B: summary for the single-channel conductance in 9 excised patch experiments showing no significant change in the mean single BK channel conductance following the application of 0.3% EtOH (N = 9, P > 0.05). C: summary data for the effects of EtOH on the open channel probability, the number of events, and the single BK channel amplitude at +40 mV. Each data-point is n = 13–16 (N = 11–13). Data in A–C were obtained with ∼300 nM free [Ca²⁺] in symmetrical 140 mM K⁺ solution. Statistically significant effects were noted for P_o and the number of events as indicated; *P < 0.05, **P < 0.01 (vs. pre-EtOH control values).