Fig. 6.

Lack of an effect by EtOH on native DSM single BK channel activity under the condition of moderately elevated intracellular [Ca²⁺]. A: representative recordings from a single experiment (inside-out configuration at +40 mV) with K⁺ solution containing ∼2 μM free [Ca²⁺] for the intracellular (bath) compartment before and after the application of 0.3% EtOH. Shown are also all point-histograms (analyzed interval of 30 s) for each condition. C depicts the closed channel state along with the dotted line and arrowheads the open channel states. B and C: summaries for the single BK channel open probability (B) and single BK channel current amplitude (C) in 11 inside-out excised patch experiments showing no significant changes in the mean values following the application of 0.3% EtOH in the presence of ∼2 μM intracellular free [Ca²⁺] (N = 8, P > 0.05); ns, nonsignificant vs. pre-EtOH control values.