Skip to main content
. 2013 Nov 6;306(1):C19–C27. doi: 10.1152/ajpcell.00156.2013

Fig. 6.

Fig. 6.

Cardiac mitochondrial function and characteristics. A: PI permeabilized cardiac fiber basal oxygen consumption supported by glutamate, malate, and pyruvate (V0), maximal oxygen consumption (ADP-stimulated) supported by glutamate, malate, and pyruvate through complex I (VMAX-CI), and maximal convergent oxygen flux supported by glutamate, malate, pyruvate, and succinate (VMAX-CI+CII). n = 8–10 mice/group. B: succinate control ratio (SCR; defined as VMAX-CI+CII/VMAX-CI). n = 9–10 mice per group. C: PI citrate synthase (CS) activity (mmol·min−1·mg protein−1). n = 8–10 mice/group. Remote LV (D) and PI (E) mitochondrial oxidative phosphorylation (OXPHOS) complexes I-V (CI-CV), nuclear respiratory factor-1 (NRF-1), mitochondrial transcription factor A (TFAM), and uncoupling protein-3 (UCP-3), as determined by immunoblotting, are shown. F: representative immunoblotting of the regional protein levels OXPHOS complexes I-V, NRF-1, TFAM, and UCP-3. Protein levels were normalized to GAPDH content and are expressed relative to the Sham group. n = 6 mice/group. Values are means ± SE. *P < 0.05 vs. Sham. †P < 0.05 vs. MI+PBS PI.