Table 1.
Primer sequences used for cloning and sequencing of Squalus acantias cGMP-dependent protein kinase
| Primer | Target | Sequence | Target |
|---|---|---|---|
| Fw1 | cGKII | ATGGGAAATGGTTCAGTGAAACCCAAAC | cGKII 1–28 |
| Fw2 | cGKII | CAAGTGCATCCAGCTGAACAAGCTGCAGGA | cGKII 222–251 |
| Fw3 | cGKII | TCCAGCTNAACAARCTKCARGA | cGKII 230–251 |
| Fw4 | cGKII | AAGAAGCTCATTACAGATGCCCTKAATAANAAYCAGT | cGKII 466–502 |
| Fw5 | cGKII | ACCAYNTCTWTGTGYTNGCAGAKGG | cGKII 608–632 |
| Fw6 | cGKII | ATYMGAGARGGNGARGAAGGARRYACCTT | cGKII 940–968 |
| Fw7 | cGKII | TTTGCYATGAARTGYATNARGAARAAGCACATHGTKGA | cGKII 1435–1472 |
| Fw8 | cGKII | TTCAAGGAYAATAAGTATGTRTACATG | cGKII 1558–1584 |
| Fw9 | cGKII | TGGAGGCCTGYYTRGGTGGRGARCTCTGGA | cGKII 1589–1618 |
| Fw10 | cGKII | ACATGGACATTCTGTGGGACTCC | cGKII 1819–1841 |
| Rv1 | cGKII | TCCTGCAGCTTGTTCAGCTGGATGCACTTG | cGKII 222–251 |
| Rv2 | cGKII | ACTGRTTNTTATTMAGGGCATCTGTAATGAGCTTCTT | cGKII 466–502 |
| Rv3 | cGKII | ACAAAGATATGGTTTCCNGGTTCTCC | cGKII 595–620 |
| Rv4 | cGKII | TGGAANACYTCTCKATCBAGNGCCCA | cGKII 763–788 |
| Rv5 | cGKII | TCMACDATGTGCTTYTTCYTNATRCAYTTCATRGCAAA | cGKII 1435–1472 |
| Rv6 | cGKII | TCATCRAARCTNCCYCTGTCYCTTARWATRSTCCA | cGKII 1615–1649 |
| Rv7 | cGKII | ACATACTCTGGAGTCCCACAGAATGTCCA | cGKII 1822–1850 |
| Rv8 | cGKII | AGAAGTCTTTRTCCCAGCCTGACANYTCATC | cGKII 2262–2287 |
| Fw11 | cGKIα | ATGAGYGANCTAGANGAAGACTTYGCSAAGATT | cGKIα 1–33 |
| Fw12 | cGKIβ | ATGGGCACCYTGCGNGAYYTNCAGTACGCGCTCCA | cGKIβ 1–34 |
| Fw13 | cGKI | CTNATMAAGGARGCYATCYTKGAYAATGACTTYATGAA | cGKIα/β |
| Fw14 | cGKI | GATGCYACSACTCGKTTYTACACKGCCTGTGTG | cGKIα/β |
| Rv9 | cGKI | GAAGCTCAGACAATCAGAAGGGCTCC | cGKIα/β |
| Rv10 | cGKI | TTGTAMGTTTTCATKGGRTCGGRRCCTGAGAACGG | cGKIα/β |
| Fw15 | cGKI and II | GGNGARCTGGCCATYYTNTAYAAYTGYACNMGRACNGC | cGMP binding domain |
| Rv11 | cGKI and II | TTNACNAGYTCNACNCKNCCRAANCCWCCSACNCCYARWGT | Kinase domain |
| Rv12 | cGKI and II | CCYTTRTTNAKDATNAYYTCNGGNGCCATRTAYTCNGGWGTNCC | Kinase domain |
| Fw17 | S. acanthias cGKI | ACAGTCAAAGCCCTCGCCAACATC | 224–247 |
| Fw16 | S. acanthias cGKI | GAGCGTACAGATTTCAATGTCTG | 688–710 |
| Fw15 | S. acanthias cGKI | GCCGGAGAACATAATCCTCA | 1080–1099 |
| Fw18 | S. acanthias cGKI | GAGGCTCTATAGGACATTCAAG | 1431–1452 |
| Rv13 | S. acanthias cGKI | GGATTGTCCCTGCACAGTTT | 1399–1418 |
| Rv14 | S. acanthias cGKI | CATCACCCTCTTTGATGATGCA | 70–91 |
| Rv18 | S. acanthias cGKI | CCTGGACAGAGAACTGGGAAGCT | 458–480 |
| Rv15 | S. acanthias cGKI | ATCGAAAGAGCCCCTGTCCCTCAG | 993–970 |
| Rv16 | S. acanthias cGKI | TCTGTATCTGTCCCTGAGAATAGTC | 1026–1050 |
| Rv19 | S. acanthias cGKI | CTTAGCATAGCCCCGGTGATCA | 1185–1206 |
| Rv17 | S. acanthias cGKI | AGGACCAGTAATCTGCGGAGC | 1232–1252 |
| Fw19 | NPR-C | GACGGACGAACGTCATTTTT | 540–559 |
| Rv18 | NPR-C | TCCAGATCAGCACTCGACAC | 985–1004 |
Positions for cGMP-dependent protein kinase II (cGKII) are relative to the start of the open reading frame of human cGKII. Positions in S. acanthias-specific primers refer to the start of the open reading frame or the start of the known sequence. NPR-C, natriuretic peptide receptor C type.